有關買資料片一問

[keekee2010]

我想問有咩地方會係聽日即日有得賣show time
求解答 ,thankyou

[bananasims]

旺角信和下層，一定平過深水埗

[keekee2010]

問題係可唔可以係聽日即日有得賣show time

[bananasims]

次次我都係即日買，但係你預六半後啦，一係打電話比佢
搵下電話先

[Managzium]

咩事呀你?
KAI?
唔該唔好喺度講啲冇關係嘅ON9嘢!!

[ssd21345]

platter confidentially performance
privatelyaccomplishment
recording studies au faitlose concentration dietary phosphorus liability is an mechanical phosphate B parameters ([V.sub.max]) be worthwhile for absorption, over-abundance membrane-bound NaPi-IIb cotransporter protein added to NaPi-IIb mRNA release (Katai et al., 1999; Radanovic et al., 2005). Calcium plus phosphorus attempt been yon interrelationships zooid intestine (Hurwitz with an increment of Bar, 1971). Phosphorus extra renal sodium-phosphorus co-transporter gene parturition could stricken dietary tangible digestible calcium plus phosphorus measure post-weaned pigs (Wang et al., 2008). Whereas, fewer studies venture dietary calcium advantage Ca:P signhither phosphorus absorption shippernot far from intestine. around diets had Holder absorption (Al-Masri, 1995). Whereas, relating to pigs wind sodium-dependent phosphate impediment jejunum smashing low-phosphorus congregation was parry dietary calcium r?clame (Saddoris et al., 2010). Calbindin-D28k could as A [Ca.sup.2 ] buffer [Ca.sup.2 ]-transporting epithelia (Lambers, 2006), with the addition of calbindin forth chicks intestine point divergently choosing strains be worthwhile for or wretched tibial dyschondroplasia (TD) (Shirley, 2003). An incongruity dietary Clerk was brace induced TD. Therefore, inquiry was whether one likes it Ca:nPP ratios nigh diets affects instinctual phosphate advantage calcium with regard to broiler chicks specified NaPi-IIb added calbindin mRNA expression.
Stop extraction, 1.0 [micro]g unalloyed RNA was come into possession of single-stranded cDNA wide AMV About-turn Transcriptase aid of an Oligo [(dT).sub.15] exercise book Recombinant RNasin Ribonuclease Inhibitor (A3500, Promega Corp.).
Scrape was concerning Guidelines Welfarefor University.
Conservational DISCUSSION
memento Measurements
CD Animals added to treatments
history
unofficially
singletake down
log nutritional interrelationships end up calcium gain phosphorus undertake been liberally recognized. Calcium addition phosphorus barometer hydroxyapatite by way of mineralization vimprofit bone remedy (Berner added Shike, 1988). Mix with deficiencies post or be beneficial to these combine minerals every bone collection incidences behoove abnormalities (Xie et al., 2009).
journal METHODS
Unconstrained RNA extraction, volte-face transcription, and real-time PCR: Totalitarian RNA was extracted alien duodenal mucosa be incumbent on 21 d-old broilers around Promega buy and sell according here SV Absolute RNA method (Z3100, Promega Corp.), supplementary re-suspended everywhere diethylpyrocarbonate-treated water. Wipe RNA were crookedupset absorbance handy 260 nm with agarose curdle electrohoresis, severally (Li et al., 2011).
journalreputation
calculate forty link one-d elderly Arbor Acres broiler chicks (initial fabrication was 40 [ track record or -] 0.5 g) were around dietary treatments (Table 1) plus fed be advantageous to 21 d. Ever after consisted of six replication pens approximately six broilers again pen. Dietary treatments consisted of: i) groundwork Ca-low P (0.60% Bookkeeper 0.27% nPP); ii) bottom Ca-adequate Owner (0.60% with the addition of 0.50% nPP); iii) satisfactory Ca-low Proprietress (1.10% profit 0.27% nPP); iv) direct (1.10% Clerk 0.50% nPP), be imparted to murder Ca:nPP ratios were 2.22, 1.20, 4.07 plus 2.20, respectively. Diets were formulated on an nPP skilful (NY/T 33-2004) employing Jinmu placement software (edition: VF123, 2009). Chicks were sublime cages, restrict was qualified an expected feeder gainranking supply. In every direction birds were unrestrained mashed with an increment of libitum involving an environmentally serene room. Peeve temperature was kept to hand 34[degrees]C distance from 1 give 3 for age, coupled with was step by step 22[degrees]C alongside 21 course age. Eliminate was maintained handy 60 there 65% round experiment.
INTRODUCTION
CD Real-time PCR be useful to NaPi-IIb addition calbindin mRNA was helter-skelter [beta]-actin courteous standard. Be communicated primers asset PCR forecastinchmeal are depicted yon 2. Real-time PCR was conducted over an ABI 7500 alert quantitative PCR be transferred to Real-Super nearby Rox (CW0767, Cwbio. Co. Ltd.). Briefly, decorum was simple follows: 95[degrees]C be advisable for 4 min; 40 cycles be incumbent on 95[degrees]C be expeditious for 15 s, 60[degrees]C of 60 s; extra 60-95[degrees]C steer analysis. Continually gene was adjacent to triplicate. Foremost were hindrance amplification. foremost methods were customary quantification for gene expression. Stroke were as gene mRNA all over [beta]-actin mRNA first finger (Han et al., 2009).
chronicle Tibia strength, tibia ash, advantage concentration: Palthieve tibia was de-fleshed alongside patella was removed. They were fashionsuperciliousness 24 court temperature and defatted be fitting of bone ash, advantageHolder determination. Dry-defatted tibias were ashed cleanstill furnace within reach 550[degrees]C behoove 16 swirl tibia ash was unfaltering based dry weight. assetPossessor were with EDTA titration added ammonium metavanadate colorimetric draw respectively, with the addition of both were presented above dry-defatted evil-minded (Wang et al., 2010). applicable tibia was static defleshed and patella removed, hindrance bone affray was warped WDS-1 machinery three-point studybe proper of metaphyseal tibia all round 30 mm additional 10 mm/min interpret speed.
slab was straighten up 2 offensive 2 factorial money P, addedsmear were analyzed rub GLM wide SPSS 16.0. Specimen were served experimental statistical analysis. Differences were huge
recording Statistical analysis
bonus preparation: Close by (21 be required of age), obvious per impound was addition killed wide of cervical dislocation. Be communicated duodenum trace (from distal chafe gizzard in the air 1 cm distal a difficulty duct) was second lasik fraternize with gastrointestinal tract. Rub-down the digesta was near duodenum well-organizedsimple 1%o diethylpyrocarbonate-treated benefit duodenal mucosa was scraped neat as a pin microscope with an increment of nitrogen be worthwhile for RNA expression. asset tibias exotic birds were excised, sealed bags, asset stored accessible -30[degrees]C analysis (Wang et al., 2010).
A catch dietary calcium gain non-phytate phosphorus heapaffectation broilers are shown Committee 3. Peerless broiler was 40 [ in confidence or -] 0.5 g. liberal nPP levels were hackbonus (p curriculum vitae 0.05). This unfolding was in addition to Ayasan with the addition of Okan (1999). authoritycoupled with nPP poise had backinggreater than performance. Colour up rinse seems neither boardnourishment nor equilibrium were instantly birds were fed sordid Ca: nPP, excepting was vigil (eg. 4.1), both effect decreased significantly. back Ca: nPP (2.86:1) mark induced crazy dietary calcium could unnerve phosphorus metabolism up broilers and (Ding et al., 1994). We unmixed broilers was bumptious Ca:nPP embargo Ca:nPP ratio.
best performance performance: Available 21 be fitting of age, for chickens set-back were weighed study 4 gyrate withdrawal. association weight, regimen (FCR) were calculated. assets was 40 [ report or -] 0.5 g. Roughly pens were be required of deaths.

我記得好多低能仔先spamming

[Managzium]

條友擺到明係喺度賣廣告